TDP-43 (C-terminal) Polyclonal antibody

• Featured Product
• KD/KO Validated

TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

239

Reviews (8)

Cat no : 12892-1-AP

Synonyms

TDP 43, TARDBP, TDP43, TDP-43, TAR DNA-binding protein 43

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Validation Data Gallery

• 

  WB analysis of A549 cells using 12892-1-AP

  A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000.

• 

  WB analysis of HeLa using 12892-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12892-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 12892-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TARDBP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12892-1-AP

  IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug.

• 

  IP experiment of mouse brain using 12892-1-AP

  IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 4ug; Detection:12892-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

• 

  IHC staining of mouse brain using 12892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12892-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12892-1-AP

  Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse brain using 12892-1-AP

  Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 12892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:2400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of Neuro-2a using 12892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Neuro-2a using 12892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12892-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (C-terminal) (12892-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: A549 cells, mouse brain tissue, HeLa cells, C6 cells
• Positive IP detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells, Neuro-2a cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 145 publications below
• IHC: See 48 publications below
• IF: See 89 publications below
• IP: See 10 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 1 publications below

Product Information

12892-1-AP targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, chicken, zebrafish, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-45 kDa, 35 kDa
• GenBank Accession Number: BC001487
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• RRID: AB_2200505
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Publications

Species	Application	Title
human	IF	Nature; TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.; Authors - Anna-Leigh Brown
human	WB,IHC	Science; HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.; Authors - Haiyang Yu ; KO Validated
human,mouse	IF	Nature; TDP-43 and RNA form amyloid-like myo-granules in regenerating muscle.; Authors - Thomas O Vogler
mouse	WB,IF	Nature; Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice.; Authors - Lindsay A Becker
human	IF	Nat Med; Childhood amyotrophic lateral sclerosis caused by excess sphingolipid synthesis.; Authors - Payam Mohassel
human	WB	Nat Cell Biol; Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition; Authors - Shan Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Scott (Verified Customer) (10-22-2024)

10µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size, blue bar, with Alpha-tubulin internal control (red band - 66031-1-Ig). Precision plus protein standard ladder #1610373.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: TDP43 Q331K Mouse Brain Lysate

TDP-43 (C-terminal) Antibody Western Blot validation (1:2500 dilution) in TDP43 Q331K Mouse Brain Lysate (Cat no:12892-1-AP)

FH

Parijat (Verified Customer) (09-09-2023)

Good antibody

• Applications: Immunofluorescence

TDP-43 (C-terminal) Antibody Immunofluorescence validation ( dilution) in (Cat no:12892-1-AP)

FH

Xin (Verified Customer) (01-23-2022)

Good performance in WB (around 45 kD)

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

TDP-43 (C-terminal) Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:12892-1-AP)

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by TDP-43 (C-terminal) Polyclonal antibody at dilution of 1:500 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: NSC-34 cells

FH

Jacob (Verified Customer) (03-25-2021)

Good for IF staining. It worked for cells fixation with 4% PFA.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Neuron

TDP-43 (C-terminal) Antibody Immunofluorescence validation (1:250 dilution) in Neuron (Cat no:12892-1-AP)

FH

David (Verified Customer) (01-13-2020)

Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500 (IF); 1:1000 (WB)
• Cell Tissue Type: SH-SY5Y

FH

Alinda (Verified Customer) (02-28-2019)

Good antibody

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: SHSY5Y

FH

Noemi (Verified Customer) (01-24-2019)

really good antibody, it detects also the cleavage product of TDP43 in WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human astrocytes

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