Tested Applications
| Positive WB detected in | A549 cells, C6 cells, mouse brain tissue, Calu-1 cells, HeLa cells, K-562 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue, human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells, Neuro-2a cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:2000-1:8000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12892-1-AP targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, chicken, zebrafish, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | TAR DNA binding protein |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43-45 kDa, 35 kDa |
| GenBank Accession Number | BC001487 |
| Gene Symbol | TDP-43 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_2200505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13148 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
| IF protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
| IHC protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
| IP protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
| WB protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-TDP-43 Rabbit Monoclonal Antibody (Cat# 12892-1-AP) has accumulated 322 citations in high-impact journals including Nature, Science, Cell, Nature Medicine, Nature Neuroscience, Nature Communications, Neuron, Molecular Neurodegeneration, Brain, and Acta Neuropathologica. Research fields span amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), TDP-43 proteinopathy, stress granule dynamics, RNA splicing, protein aggregation, Alzheimer's disease, mitochondrial dysfunction, autophagy, and neuroinflammation.
| Species | Application | Title |
|---|---|---|
Nature Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice. | ||
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Science Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity. |
Reviews
What customers say
Users consistently rate this antibody highly (mostly 5/5) across Western blot, immunofluorescence, and immunohistochemistry. It detects TDP43 at the expected ~45 kDa size with clear specificity in diverse cell types including SH-SY5Y, iPSC-derived motor neurons, NSC-34, neurons, and human astrocytes. Several reviewers highlight its ability to detect stress-induced C-terminal cleavage fragments. It performs well with 4% PFA fixation at dilutions ranging from 1:200 to 1:2500. Overall, users describe it as sensitive, specific, and reliable for both intact protein and fragment detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emilie (Verified Customer) (09-24-2025) | I used it both in WB (1:1000) and IF (1:200), and it performed as expected.
|
Manon (Verified Customer) (09-23-2025) | The antibody produced clear, specific staining by immunofluorescence and also detected the protein at the expected size by western blot.
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Xiaochen (Verified Customer) (11-11-2024) | sensitive and specific
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Scott (Verified Customer) (10-22-2024) | 10µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size, blue bar, with Alpha-tubulin internal control (red band - 66031-1-Ig). Precision plus protein standard ladder #1610373.
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Parijat (Verified Customer) (09-09-2023) | Good antibody
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Xin (Verified Customer) (01-23-2022) | Good performance in WB (around 45 kD)
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Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by TDP-43 (C-terminal) Polyclonal antibody at dilution of 1:500 showed strong labelling.
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Jacob (Verified Customer) (03-25-2021) | Good for IF staining. It worked for cells fixation with 4% PFA.
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David (Verified Customer) (01-13-2020) | Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.
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Alinda (Verified Customer) (02-28-2019) | Good antibody
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Noemi (Verified Customer) (01-24-2019) | really good antibody, it detects also the cleavage product of TDP43 in WB.
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