TDP-43 (N-terminal) Recombinant antibody, PBS Only (Detector)

• Featured Product
• KD/KO Validated

TDP-43 (N-terminal) Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

16A22

Cat no : 80002-1-PBS

Synonyms

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

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Validation Data Gallery

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  WB analysis using 80002-1-RR (same clone as 80002-1-PBS)

  Various cell lysates were subjected to SDS PAGE followed by western blot with 80002-1-RR (TDP-43 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

  HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80002-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

  WB result of TDP-43 antibody (80002-1-RR; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IP experiment of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

  HAP1 lysates prepared and IP of TARDBP performed using 2.0 μg of 80002-1-RR coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

  IP result of anti-TDP-43 (N-terminal) (IP:80002-1-RR, 4ug; Detection:80002-1-RR 1:20000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80002-1-RR (same clone as 80002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RREC80002 (TDP-43 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80002-1-RR (same clone as 80002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80002-1-RR (TDP-43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 80002-1-RR (same clone as 80002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 80002-1-RR (same clone as 80002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IF Staining of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

  HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80002-1-RR at 1:200 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80002-1-RR (same clone as 80002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 80002-1-RR (TDP-43 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (for IF/FC) (80002-1-RR, Clone:16A22) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP00589-2

  Cytometric bead array in cell lysate using MP00589-2, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 80002-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

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  Affinity and Kinetic Characterization of 80002-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80002-1-RR against Human TDP-43 (N-terminal) were performed. The affinity constant is below 1 pM.

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Product Information

80002-1-PBS targets TDP-43 (N-terminal) as part of a matched antibody pair:

MP00589-2: 80007-1-PBS capture and 80002-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TDP-43 (N-terminal) fusion protein Ag1231
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC001487
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• RRID: AB_2882934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)

80002-1-RR antibody works well in IF experiment.