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  • Featured Product
  • KD/KO Validated

TDP-43 Recombinant antibody

TDP-43 Recombinant Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

16A22

Cat no : 80002-1-RR

Synonyms

ALS10, TAR DNA binding protein 43, TARDBP, tdp 43, TDP43, TDP-43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDP-43 Recombinant antibody (80002-1-RR)

    WB analysis using 80002-1-RR

    Various cell lysates were subjected to SDS PAGE followed by western blot with 80002-1-RR (TDP-43 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HAP1 using TDP-43 Recombinant antibody (80002-1-RR)

    WB analysis of HAP1 using 80002-1-RR

    HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80002-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Western Blot (WB) analysis of HeLa cells using TDP-43 Recombinant antibody (80002-1-RR)

    WB analysis of HeLa using 80002-1-RR

    WB result of TDP-43 antibody (80002-1-RR; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HAP1 using TDP-43 Recombinant antibody (80002-1-RR)

    IP experiment of HAP1 using 80002-1-RR

    HAP1 lysates prepared and IP of TARDBP performed using 2.0 μg of 80002-1-RR coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TDP-43 Recombinant antibody (80002-1-RR)

    IHC staining of mouse brain using 80002-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80002-1-RR (TDP-43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HAP1 using TDP-43 Recombinant antibody (80002-1-RR)

    IF Staining of HAP1 using 80002-1-RR

    HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80002-1-RR at 1:200 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using TDP-43 Recombinant antibody (80002-1-RR)

    IF Staining of rat brain using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using TDP-43 Recombinant antibody (80002-1-RR)

    IF Staining of SH-SY5Y using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TDP-43 Recombinant antibody (80002-1-RR)

    IF Staining of HeLa using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using TDP-43 Recombinant antibody (80002-1-RR)

    IF Staining of rat brain using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TDP-43 Recombinant antibody (80002-1-RR)

    IF Staining of HepG2 using 80002-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 80002-1-RR (TDP-43 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

  • Flow cytometry (FC) experiment of HeLa cells using TDP-43 Recombinant antibody (80002-1-RR)

    FC experiment of HeLa using 80002-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (for IF/FC) (80002-1-RR, Clone:16A22) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HAP1, K-562 cells, Neuro-2a cells, C6 cells
Positive IP detected inHAP1 cells
Positive IF detected inSH-SY5Y cells, rat brain tissue, HAP1 cells, HeLa cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80002-1-RR targets TDP-43 as part of a matched antibody pair:

MP00589-2: 80007-1-RR capture and 80002-1-RR detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TDP-43 fusion protein Ag1231
Full Name TAR DNA binding protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TARDBP
Gene ID (NCBI) 23435
RRIDAB_2882934
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)

80002-1-RR antibody works well in IF experiment.

Protocols

Product Specific Protocols
WB protocol for TDP-43 antibody 80002-1-RRDownload protocol
IHC protocol for TDP-43 antibody 80002-1-RRDownload protocol
IF protocol for TDP-43 antibody 80002-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Neural Regen Res

5-Hydroxytryptamine: a potential therapeutic target in amyotrophic lateral sclerosis

Authors - Shi-Shi Jiang
View Article
humanWB,IF,IP

F1000Res

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence

Authors - Donovan Worrall
View Article
humanIHC

J Neuropathol Exp Neurol

COVID-19 could worsen cerebral amyloid angiopathy

Authors - Julie Dewisme
View Article