TCP11L2 Recombinant antibody

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  WB analysis using 83227-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 83227-1-RR

  MDCK cells were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 83227-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TCP11L2 antibody (83227-1-RR, Clone: 240096F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of A431 using 83227-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of SH-SY5Y using 83227-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83227-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human TCP11L2 were performed. The affinity constant is below 1 pM.

TCP11L2 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83227-1-RR

Clone No.240096F6

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6

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Tested Applications

• Positive WB detected in: Jurkat cells, MDCK cells, U251 cells
• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: A431 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83227-1-RR targets TCP11L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TCP11L2 fusion protein Ag11185
• Full Name: t-complex 11 (mouse)-like 2
• Calculated Molecular Weight: 519 aa, 58 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC033617
• Gene Symbol: TCP11L2
• Gene ID (NCBI): 255394
• RRID: AB_3670910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N4U5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087)