CoraLite® Plus 488-conjugated TCF7L1/TCF7L2 Monoclonal antibody

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  FC experiment of A431 using CL488-67652

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TCF7L1/TCF7L2 (CL488-67652, Clone:2B2D12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TCF7L1/TCF7L2 Monoclonal Antibody for FC (Intra)

Cat No. CL488-67652

Clone No.2B2D12

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

FC (Intra)

Synonyms

TCF7L1, TCF7L2, TCF4, TCF-3, TCF3

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Tested Applications

• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67652 targets TCF7L1/TCF7L2 in FC (Intra) applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TCF7L1/TCF7L2 fusion protein Ag28954
• Full Name: transcription factor 7-like 1 (T-cell specific, HMG-box)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC058894
• Gene Symbol: TCF7L1
• Gene ID (NCBI): 83439
• RRID: AB_2934557
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HCS4
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. This antibody raised against 1-200aa of human TCF7L1 can also react with TCF7L2.