TBCC Monoclonal antibody, PBS Only

TBCC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B7D9

Cat no : 68371-1-PBS

Synonyms

Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC, 2B7D9

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Validation Data Gallery

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  WB analysis using 68371-1-Ig (same clone as 68371-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Calu-3 using 68371-1-Ig (same clone as 68371-1-PBS)

  Calu-3 cells were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68371-1-Ig (same clone as 68371-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TBCC antibody (68371-1-Ig, Clone: 2B7D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

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Product Information

68371-1-PBS targets TBCC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBCC fusion protein Ag8962
• Full Name: tubulin folding cofactor C
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC020170
• Gene Symbol: TBCC
• Gene ID (NCBI): 6903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TBCC, also named CFC. Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level). Cofactor C was cytoplasmic in distribution (PMID: 12417528).