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TBCC Monoclonal antibody

TBCC Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7D9

Cat no : 68371-1-Ig

Synonyms

Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC, 2B7D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBCC Monoclonal antibody (68371-1-Ig)

    WB analysis using 68371-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of Calu-3 cells using TBCC Monoclonal antibody (68371-1-Ig)

    WB analysis of Calu-3 using 68371-1-Ig

    Calu-3 cells were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBCC Monoclonal antibody (68371-1-Ig)

    IF Staining of HeLa using 68371-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TBCC antibody (68371-1-Ig, Clone: 2B7D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHT-1376 cells, Calu-3 cells, HeLa cells, MDA-MB-231 cells, MDA-MB-468 cells, HepG2 cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68371-1-Ig targets TBCC in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TBCC fusion protein Ag8962
Full Name tubulin folding cofactor C
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC020170
Gene Symbol TBCC
Gene ID (NCBI) 6903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBCC, also named CFC. Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level). Cofactor C was cytoplasmic in distribution (PMID: 12417528).

Protocols

Product Specific Protocols
WB protocol for TBCC antibody 68371-1-IgDownload protocol
IF protocol for TBCC antibody 68371-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols