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Biotin-conjugated TDP-43 (N-terminal) Polyclonal antibody
TDP-43 (N-terminal) Polyclonal Antibody for IHC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, zebrafish
Applications
IHC
Conjugate
Biotin
Cat no : Biotin-10782
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
Biotin-10782 targets TDP-43 (N-terminal) in IHC applications and shows reactivity with human, mouse, rat, zebrafish samples.
Tested Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | fusion protein |
Full Name | TAR DNA binding protein |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 44 kDa |
GenBank Accession Number | BC001487 |
Gene Symbol | TDP-43 |
Gene ID (NCBI) | 23435 |
RRID | AB_2918934 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
Protocols
Product Specific Protocols | |
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IHC protocol for Biotin TDP-43 (N-terminal) antibody Biotin-10782 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |