TACSTD2/TROP2 Monoclonal antibody

TACSTD2/TROP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5A6B1

Cat no : 68141-1-Ig

Synonyms

EGP 1, GA733, GA733 1, M1S1, TACSTD2, TROP2



Tested Applications

Positive WB detected inHaCaT cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68141-1-Ig targets TACSTD2/TROP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TACSTD2/TROP2 fusion protein Ag25775
Full Name tumor-associated calcium signal transducer 2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC009409
Gene Symbol TROP2
Gene ID (NCBI) 4070
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).

Protocols

Product Specific Protocols
WB protocol for TACSTD2/TROP2 antibody 68141-1-IgDownload protocol
IHC protocol for TACSTD2/TROP2 antibody 68141-1-IgDownload protocol
IF protocol for TACSTD2/TROP2 antibody 68141-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols