TAB1 Monoclonal antibody, PBS Only

TAB1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E10D12

Cat no : 67020-1-PBS

Synonyms

3' Tab1, MAP3K7IP1, TAB1, TAK1 binding protein 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 67020-1-Ig (same clone as 67020-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67020-1-Ig (same clone as 67020-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67020-1-Ig (same clone as 67020-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67020-1-Ig (same clone as 67020-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67020-1-Ig (same clone as 67020-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67020-1-Ig (same clone as 67020-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67020-1-Ig (same clone as 67020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67020-1-Ig (same clone as 67020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67020-1-Ig (same clone as 67020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TAB1 antibody (67020-1-Ig, Clone: 1E10D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67020-1-PBS targets TAB1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TAB1 fusion protein Ag6590
• Full Name: mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC050554
• Gene Symbol: MAP3K7IP1
• Gene ID (NCBI): 10454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.