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TAB1 Monoclonal antibody

TAB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E10D12

Cat no : 67020-1-Ig

Synonyms

MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TAB1 Monoclonal antibody (67020-1-Ig)

    WB analysis of HEK-293 using 67020-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TAB1 Monoclonal antibody (67020-1-Ig)

    WB analysis of K-562 using 67020-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using TAB1 Monoclonal antibody (67020-1-Ig)

    WB analysis of HSC-T6 using 67020-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using TAB1 Monoclonal antibody (67020-1-Ig)

    WB analysis of NIH/3T3 using 67020-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TAB1 Monoclonal antibody (67020-1-Ig)

    WB analysis of HeLa using 67020-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TAB1 Monoclonal antibody (67020-1-Ig)

    WB analysis of MCF-7 using 67020-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TAB1 Monoclonal antibody (67020-1-Ig)

    IHC staining of human lung cancer using 67020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TAB1 Monoclonal antibody (67020-1-Ig)

    IHC staining of human lung cancer using 67020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TAB1 Monoclonal antibody (67020-1-Ig)

    IF Staining of A431 using 67020-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TAB1 antibody (67020-1-Ig, Clone: 1E10D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HSC-T6 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67020-1-Ig targets TAB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TAB1 fusion protein Ag6590
Full Name mitogen-activated protein kinase kinase kinase 7 interacting protein 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC050554
Gene Symbol TAB1
Gene ID (NCBI) 10454
RRIDAB_2882336
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TAB1 antibody 67020-1-IgDownload protocol
IHC protocol for TAB1 antibody 67020-1-IgDownload protocol
IF protocol for TAB1 antibody 67020-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mutat

The MAP3K7 gene: further delineation of clinical characteristics and genotype/phenotype correlations.

Authors - Geeske M van Woerden
View Article
humanWB,IP

Leukemia

Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15

Authors - Cong Liu
View Article