CoraLite® Plus 488-conjugated Synaptophysin Recombinant antibody

Synaptophysin Uni-rAb^TM Recombinant Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H14

Cat no : CL488-82900

Synonyms

SYP, SYN, MRX96, 2H14

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-82900

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-82900, Clone: 2H14 ) at dilution of 1:200, Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using CL488-82900

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 Synaptophysin antibody (CL488-82900, Clone: 2H14 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82900 targets Synaptophysin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Synaptophysin fusion protein Ag11781
• Full Name: synaptophysin
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC064550
• Gene Symbol: Synaptophysin
• Gene ID (NCBI): 6855
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)