Synaptophysin Recombinant antibody, PBS Only

Synaptophysin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H14

Cat no : 82900-1-PBS

Synonyms

SYP, SYN, MRX96, 2H14

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Validation Data Gallery

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  WB analysis using 82900-1-RR (same clone as 82900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82900-1-RR (Synaptophysin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 82900-1-RR (same clone as 82900-1-PBS)

  IP result of anti-Synaptophysin (IP:82900-1-RR, 4ug; Detection:82900-1-RR 1:5000) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82900-1-RR (same clone as 82900-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Synaptophysin antibody (82900-1-RR, Clone: 2H14 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 82900-1-RR (same clone as 82900-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Synaptophysin Recombinant antibody (82900-1-RR, Clone:2H14) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82900-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82900-1-RR against Human Synaptophysin were performed. The affinity constant is below 1 pM.

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Product Information

82900-1-PBS targets Synaptophysin in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Synaptophysin fusion protein Ag11781
• Full Name: synaptophysin
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC064550
• Gene Symbol: Synaptophysin
• Gene ID (NCBI): 6855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)