Stathmin 1 Polyclonal antibody, PBS Only

• 

  WB analysis of Jurkat using 11157-1-AP (same clone as 11157-1-PBS)

  WB results of Stathmin antibody (11157-1-AP, 1:20000) with si-control and si-Stathmin transfected Jurkat cells.. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11157-1-AP (same clone as 11157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 11157-1-AP (same clone as 11157-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11157-1-AP (Stathmin 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11157-1-AP (same clone as 11157-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Stathmin 1 antibody (11157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 11157-1-AP (same clone as 11157-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Stathmin 1 (11157-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Stathmin 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 11157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

STMN1, C1orf215, LAP18, Leukemia-associated phosphoprotein p18, OP18

---

Product Information

11157-1-PBS targets Stathmin 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Stathmin 1 fusion protein Ag1633
• Full Name: stathmin 1/oncoprotein 18
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC014353
• Gene Symbol: Stathmin 1
• Gene ID (NCBI): 3925
• RRID: AB_2197114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16949
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.