Stathmin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Stathmin 1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

25

Reviews (1)

Cat no : 11157-1-AP

Synonyms

STMN1, stathmin 1/oncoprotein 18, Stathmin, OP18, Leukemia-associated phosphoprotein p18

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Validation Data Gallery

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  WB analysis of Jurkat using 11157-1-AP

  WB results of Stathmin antibody (11157-1-AP, 1:20000) with si-control and si-Stathmin transfected Jurkat cells..

• 

  WB analysis using 11157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11157-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11157-1-AP (Stathmin 1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 11157-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Stathmin 1 (11157-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, PC-12 cells
• Positive IHC detected in: human gliomas tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 8 publications below
• IF: See 3 publications below

Product Information

11157-1-AP targets Stathmin 1 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Stathmin 1 fusion protein Ag1633
• Full Name: stathmin 1/oncoprotein 18
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC014353
• Gene Symbol: Stathmin 1
• Gene ID (NCBI): 3925
• RRID: AB_2197114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Stem Cell; Continuous clonal labeling reveals uniform progenitor potential in the adult exocrine pancreas.; Authors - Sophie C Lodestijn
rat	WB	Chemosphere; Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.; Authors - Mingdan You
human	WB	Int J Nanomedicine; Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.; Authors - Chen Li
human	WB	Oncogene; KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.; Authors - Lei Zhao ; KD Validated
human	IHC	Oncotarget; Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients.; Authors - Xia-Ying Kuang
human	IHC	Neuroimage; Developmental dynamics of the periventricular parietal crossroads of growing cortical pathways in the fetal brain - In vivo fetal MRI with histological correlation.; Authors - Ruxandra-Iulia Milos

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Richard (Verified Customer) (10-08-2019)

Antibody works well at 1:4000 and gives a single band, have no issues with it.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: HeLa

Stathmin 1 Antibody Western Blot, validation (1:4000 dilution) in HeLa (Cat no:11157-1-AP)

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