ChromoTek Spot-Trap® Magnetic Particles M-270

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

Spot-Tag®

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Cat no : etd

Synonyms

etd, etd-10, etd-100, etd-20, etd-200, etd-400, Spot Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270, etd-10, etd-100, etd-20, etd-200, etd-400, PDRVRAVSHWSS, spot, spot tag, spot-tag, Spot-Trap® Magnetic Particles M-270



Product Information

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

DescriptionImmunoprecipitation of Spot-tagged proteins and their interacting factors with anti-Spot Nanobody conjugated to Magnetic Particles M-270.

• Magnetic separation with easy and efficient washing of Spot-Trap Magnetic Particles M-270

• IP and Co-IP of Spot-tagged proteins without size limitation

• No contaminating heavy and light antibody chains

• Suitable for downstream mass spec analysis

• Recombinantly expressed without batch-to-batch variation

• Extraordinary low background compared to other affinity resins

• Ready-to-use

• Short incubation time of 30-60 minutes

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetSpot-Tag® sequence PDRVRAVSHWSS
Binding capacity2.25 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Wash buffer compatibility2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 6 nM
Compatibility with mass spectrometryThe Spot-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2861252
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!