ChromoTek Spot-Label® Alexa Fluor® 647

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.

Specificity

Spot-Tag®

Applications

IF

Conjugate

Alexa Fluor® 647

Host

Alpaca

Cat no : ebAF647

Synonyms

PDRVRAVSHWSS, Spot, Spot tag, Spot-Label, SPOT-tag



Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.

DescriptionImmunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

• Capture and detection tag without compromises in applications

• Higher image resolution

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Strong avidity effect from bivalent form of Spot-VHH

• Fulfills highest requirements on antibody validation: structure and function characterized

ApplicationsIF
HostAlpaca
Specificity/TargetSpot-Tag® (PDRVRAVSHWSS)
ConjugateAlexa Fluor® 647
Physical StateLiquid
Suggested DilutionIF: 1:800
Type Nanobody
ClassRecombinant
RRIDAB_2889376
Affinity (KD) Dissociation constant KD of 6 nM
DOL1 fluorophore per bivalent Nanobody, site-directed conjugation
Storage BufferPBS, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use