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ChromoTek Spot-Label® Alexa Fluor® 568

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

Specificity

Spot-Tag®

Applications

IF

Conjugate

Alexa Fluor® 568

Host

Alpaca

Cat no : ebAF568

Synonyms

PDRVRAVSHWSS, Spot, Spot tag, Spot-Label, SPOT-tag

Validation Data Gallery

  • SPOT tag related products

    SPOT tag related products

  • Left: HeLa cells transiently expressing pSpot Spot-Tag?-Actin (ev-31) were immunostained with Spot-Label? Alexa Fluor? 568 (red, ebAF568, 1:800) and DAPI (blue). Right: Control staining with Spot-Label? Alexa Fluor? 568 (red, ebAF568, 1:800) and DAPI (blue) of untransfected HeLa cells showing no unspecific background. Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective.

    Left: HeLa cells transiently expressing pSpot Spot-Tag?-Actin (ev-31) were immunostained with Spot-Label? Alexa Fluor? 568 (red, ebAF568, 1:800) and DAPI (blue). Right: Control staining with Spot-Label? Alexa Fluor? 568 (red, ebAF568, 1:800) and DAPI (blue) of untransfected HeLa cells showing no unspecific background. Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective.

Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

DescriptionImmunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

• Capture and detection tag without compromises in applications

• Higher image resolution

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Strong avidity effect from bivalent form of Spot-VHH

• Fulfills highest requirements on antibody validation: structure and function characterized

ApplicationsIF
HostAlpaca
Specificity/TargetSpot-Tag® (PDRVRAVSHWSS)
ConjugateAlexa Fluor® 568
Physical StateLiquid
Suggested DilutionIF: 1:800
Type Nanobody
ClassRecombinant
RRIDAB_2889375
Affinity (KD) Dissociation constant KD of 6 nM
DOL1 fluorophore per bivalent Nanobody, site-directed conjugation
Storage BufferPBS, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use
Spot-Tag® OriginEngineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
Cross ReactivityThe Spot-Nanobody shows minimal cross reactivity with beta-catenin.

Publications

ApplicationTitle
IF

PLoS Genet

The chromatin remodeling protein CHD-1 and the EFL-1/DPL-1 transcription factor cooperatively down regulate CDK-2 to control SAS-6 levels and centriole number.

Authors - Jyoti Iyer
View Article
IF

bioRxiv

Polo-like kinase 1 prevents excess microtubule polymerization in C. elegans oocytes to ensure faithful meiosis

Authors - Juhi G Narula
View Article