ChromoTek Spot-Label® Alexa Fluor® 488

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.

Specificity

Spot-Tag^®

Applications

IF

Conjugate

Alexa Fluor® 488

Host

Alpaca

Publications

1

Cat no : ebAF488

Synonyms

PDRVRAVSHWSS, Spot, Spot tag, Spot-Label, SPOT-tag

---

Validation Data Gallery

• 

  SPOT tag related products

• 

  Left: HeLa cells transiently expressing Spot-Tag?-Lamin B1 were immunostained with Spot-Label? Alexa Fluor? 488 (ebAF488, 1:800). Right: Control staining of untransfected cells. Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective

---

Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.

• Description: Immunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

  • Capture and detection tag without compromises in applications

  • Higher image resolution

  • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

  • Strong avidity effect from bivalent form of Spot-VHH

  • Fulfills highest requirements on antibody validation: structure and function characterized

• Applications: IF
• Host: Alpaca
• Specificity/Target: Spot-Tag^® (PDRVRAVSHWSS)
• Conjugate: Alexa Fluor® 488
• Physical State: Liquid
• Suggested Dilution: IF: 1:800
• Type: Nanobody
• Class: Recombinant
• RRID: AB_2889374
• Affinity (KD): Dissociation constant K_D of 6 nM
• DOL: 1 fluorophore per bivalent Nanobody, site-directed conjugation
• Storage Buffer: PBS, 5 mM EDTA, preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
• Note: This product is for research use only, not for diagnostic or therapeutic use
• Spot-Tag® Origin: Engineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
• Cross Reactivity: The Spot-Nanobody shows minimal cross reactivity with beta-catenin.

Publications

Application	Title
IF	EMBO Rep; The kinase ZYG-1 phosphorylates the cartwheel protein SAS-5 to drive centriole assembly in C. elegans; Authors - Prabhu Sankaralingam