CoraLite® Plus 647-conjugated Sortilin Polyclonal antibody

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  IF Staining of mouse brain using CL647-12369

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL647-12369

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

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  FC experiment of Neuro-2a using CL647-12369

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Sortilin (CL647-12369) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Sortilin Polyclonal Antibody for IF-P, FC (Intra)

Cat No. CL647-12369

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Synonyms

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

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Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: Neuro-2a cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-12369 targets Sortilin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sortilin fusion protein Ag2997
• Full Name: sortilin 1
• Calculated Molecular Weight: 831 aa, 92 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC023542
• Gene Symbol: Sortilin
• Gene ID (NCBI): 6272
• ENSEMBL Gene ID: ENSG00000134243
• RRID: AB_2920221
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99523
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.