Sortilin Polyclonal antibody

• Featured Product
• KD/KO Validated

Sortilin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 12369-1-AP

Synonyms

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

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Validation Data Gallery

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  WB analysis using 12369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12369-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12369-1-AP

  IP result of anti-Sortilin (IP:12369-1-AP, 4ug; Detection:12369-1-AP 1:500) with rat brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 12369-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12369-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12369-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12369-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12369-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12369-1-AP (Sortilin antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 12369-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12369-1-AP (Sortilin antibody at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse brain using 12369-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12369-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Neuro-2a using 12369-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human Sortilin (12369-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Product Information

12369-1-AP targets Sortilin in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sortilin fusion protein Ag2997
• Full Name: sortilin 1
• Calculated Molecular Weight: 831 aa, 92 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC023542
• Gene Symbol: Sortilin
• Gene ID (NCBI): 6272
• RRID: AB_2302242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Evidence for sortilin modulating regional accumulation of human tau prions in transgenic mice.; Authors - Noah R Johnson
human	IF,WB	J Cell Biol; CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis.; Authors - Stéphane M Camus
mouse	WB,IF	PLoS Pathog; Prions amplify through degradation of the VPS10P sorting receptor sortilin.; Authors - Keiji Uchiyama ; KO Validated
human	WB	EBioMedicine; Neurotensin contributes to pediatric intestinal failure-associated liver disease via regulating intestinal bile acids uptake.; Authors - Yongtao Xiao ; KD Validated
human	WB	J Proteome Res; Mapping of the N-linked glycoproteome of human spermatozoa.; Authors - Wang Gaigai G
human,mouse	WB,IHC,IF,CoIP	FASEB J; Sortilin acts as an endocytic receptor for α-synuclein fibril; Authors - Shun Ishiyama

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Renato (Verified Customer) (02-27-2019)

Produces a simple clear band and the expected molecular weight. Used as diluent 5% BSA.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue

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