Sortilin Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Sortilin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12369-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12369-1-AP (same clone as 12369-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12369-1-AP (same clone as 12369-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

  IP result of anti-Sortilin (IP:12369-1-AP, 4ug; Detection:12369-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12369-1-AP (same clone as 12369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12369-1-AP (Sortilin antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12369-1-AP (same clone as 12369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12369-1-AP (Sortilin antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Neuro-2a using 12369-1-AP (same clone as 12369-1-PBS)

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human Sortilin (12369-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

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Product Information

12369-1-PBS targets Sortilin in WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sortilin fusion protein Ag2997
• Full Name: sortilin 1
• Calculated Molecular Weight: 831 aa, 92 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC023542
• Gene Symbol: Sortilin
• Gene ID (NCBI): 6272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.