CoraLite® Plus 647 Anti-Human Siglec-8 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using CL647-98177

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human Siglec-8 Rabbit RecAb (CL647-98177, Clone: 241275A5) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were co-stained with FITC Plus Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (FITC-65612, Clone: 3G8). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL647-98177

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human Siglec-8 Rabbit RecAb (CL647-98177, Clone: 241275A5) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

Siglec-8 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98177

Clone No.241275A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIGLEC8, SIGLEC 8, Sialoadhesin family member 2, Sialic acid-binding Ig-like lectin 8, SAF2

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98177 targets Siglec-8 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: sialic acid binding Ig-like lectin 8
• Calculated Molecular Weight: 499 aa, 54 kDa
• GenBank Accession Number: BC053319
• Gene Symbol: SIGLEC8
• Gene ID (NCBI): 27181
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NYZ4
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Sialic acid-binding Ig-like lectin 8 (Siglec-8), also known as SAF2, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 10856141). Siglec-8 is a single-pass transmembrane protein containing three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail (PMID: 10625619). It is an inhibitory receptor selectively expressed on human mast cells and eosinophils, and at low levels on basophils (PMID: 10856141; 33374255). Engagement of Siglec-8 with antibody results in inhibiting IgE-mediated mast cell responses and reducing allergic inflammation (PMID: 32814824).