Anti-Human Siglec-8 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98177-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-8 Rabbit Recombinant Antibody (98177-1-RR, Clone: 241275A5) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98177-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-8 Rabbit Recombinant Antibody (98177-1-RR, Clone: 241275A5) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

Siglec-8 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98177-1-RR

Clone No.241275A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIGLEC8, SIGLEC 8, Sialoadhesin family member 2, Sialic acid-binding Ig-like lectin 8, SAF2

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98177-1-RR targets Siglec-8 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: sialic acid binding Ig-like lectin 8
• Calculated Molecular Weight: 499 aa, 54 kDa
• GenBank Accession Number: BC053319
• Gene Symbol: SIGLEC8
• Gene ID (NCBI): 27181
• RRID: AB_3672320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NYZ4
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Sialic acid-binding Ig-like lectin 8 (Siglec-8), also known as SAF2, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 10856141). Siglec-8 is a single-pass transmembrane protein containing three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail (PMID: 10625619). It is an inhibitory receptor selectively expressed on human mast cells and eosinophils, and at low levels on basophils (PMID: 10856141; 33374255). Engagement of Siglec-8 with antibody results in inhibiting IgE-mediated mast cell responses and reducing allergic inflammation (PMID: 32814824).