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CoraLite® Plus 647-conjugated Septin 9 Polyclonal antibody
Septin 9 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-10769
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-10769 targets Septin 9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Septin 9 fusion protein Ag1218 |
Full Name | septin 9 |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 65 kDa, 38-40 kDa |
GenBank Accession Number | BC021192 |
Gene Symbol | SEPT9 |
Gene ID (NCBI) | 10801 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Septin 9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC). this antibody can identify all of the isoforms about SEPT9.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 Septin 9 antibody CL647-10769 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |