Septin 9 Polyclonal antibody

• Featured Product
• KD/KO Validated

Septin 9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

29

Cat no : 10769-1-AP

Synonyms

SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein

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Validation Data Gallery

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  WB analysis using 10769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 10769-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10769-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10769-1-AP

  IP result of anti-Septin 9 (IP:10769-1-AP, 4ug; Detection:10769-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10769-1-AP (Septin 9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10769-1-AP (Septin 9 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE cells using 10769-1-AP

  IF result of anti-Septin 9 (10769-1-AP, 1:200) with hTERT-RPE cell by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Green: SEPT9. Blue: DNA. Fixation in methanol.

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  IF Staining of HepG2 using 10769-1-AP

  Immunofluorescent analysis of HepG2 cells, using Septin 9 antibody 10769-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of A431 using 10769-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10769-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10769-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Septin 9 (10769-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: C6 cells, HeLa cells, mouse spleen tissue, mouse brain tissue, mouse thymus tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human colon tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, hTERT-RPE cells, HepG2 cells, A431 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 16 publications below

Product Information

10769-1-AP targets Septin 9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Septin 9 fusion protein Ag1218
• Full Name: septin 9
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 65 kDa, 38-40 kDa
• GenBank Accession Number: BC021192
• Gene Symbol: SEPT9
• Gene ID (NCBI): 10801
• RRID: AB_2185012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

Publications

Species	Application	Title
rat	IF	Dev Cell; Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.; Authors - Eva P Karasmanis ; KD Validated
canine	IF	Curr Biol; A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.; Authors - Eva P Karasmanis ; KD Validated
human	WB,IF	Curr Biol; Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.; Authors - Joëlle Salameh
human	WB, IF	J Cell Biol; A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.; Authors - Ilona A Kesisova
human	IF	J Cell Biol; Septins suppress the release of vaccinia virus from infected cells.; Authors - Julia Pfanzelter
mouse	WB	PLoS Genet; Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.; Authors - Manoj B Menon

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