Tested Applications
Positive IF/ICC detected in | MDCK cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66542 targets Septin 7 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Septin 7 fusion protein Ag4309 Predict reactive species |
Full Name | septin 7 |
Calculated Molecular Weight | 51 kDa |
Observed Molecular Weight | 48-51 kDa |
GenBank Accession Number | BC025987 |
Gene Symbol | Septin 7 |
Gene ID (NCBI) | 989 |
RRID | AB_3672913 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q16181 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. Septin 7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Septin 7 antibody CL488-66542 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |