CoraLite® Plus 488-conjugated Securin Recombinant antibody

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  IF Staining of HepG2 using CL488-83981-4

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Securin antibody (CL488-83981-4, Clone: 241090G10 ) at dilution of 1:200.

Securin Recombinant Antibody for IF/ICC

Cat No. CL488-83981-4

Clone No.241090G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83981-4 targets Securin in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Securin fusion protein Ag12670
• Full Name: pituitary tumor-transforming 1
• Calculated Molecular Weight: 202 aa, 22 kDa
• GenBank Accession Number: BC101834
• Gene Symbol: Securin
• Gene ID (NCBI): 9232
• RRID: AB_3673330
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95997
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.