Securin Recombinant antibody

Securin Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241090G10

Cat no : 83981-4-RR

Synonyms

PTTG1, PTTG, Pituitary tumor-transforming gene 1 protein, hPTTG, Esp1-associated protein



Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells, A431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83981-4-RR is Securin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Securin fusion protein Ag12670
Full Name pituitary tumor-transforming 1
Calculated Molecular Weight 202 aa, 22 kDa
GenBank Accession NumberBC101834
Gene Symbol Securin
Gene ID (NCBI) 9232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

Protocols

Product Specific Protocols
IF protocol for Securin antibody 83981-4-RRDownload protocol
Standard Protocols
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