Securin Recombinant antibody, PBS Only (Capture/Detector)

Securin Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241090G10

Cat no : 83981-4-PBS

Synonyms

PTTG1, PTTG, Pituitary tumor-transforming gene 1 protein, hPTTG, Esp1-associated protein

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Validation Data Gallery

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  IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 83981-4-RR (same clone as 83981-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83981-4-RR (same clone as 83981-4-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00876-1

  Cytometric bead array standard curve of MP00876-1, Securin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83981-2-PBS. Detection antibody: 83981-4-PBS. Standard: Ag12670. Range: 0.78-100 ng/mL.

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  Cytometric bead array standard curve of MP00876-2

  Cytometric bead array standard curve of MP00876-2, Securin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83981-1-PBS. Detection antibody: 83981-4-PBS. Standard: Ag12670. Range: 1.56-100 ng/mL.

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  Cytometric bead array standard curve of MP00876-3

  Cytometric bead array standard curve of MP00876-3, Securin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83981-4-PBS. Detection antibody: 83981-3-PBS. Standard: Ag12670. Range: 1.56-100 ng/mL.

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  Affinity and Kinetic Characterization of 83981-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83981-4-RR against Human Securin were performed. The affinity constant is 0.155 nM.

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Product Information

83981-4-PBS targets Securin as part of a matched antibody pair:

MP00876-1: 83981-2-PBS capture and 83981-4-PBS detection (validated in Cytometric bead array)

MP00876-2: 83981-1-PBS capture and 83981-4-PBS detection (validated in Cytometric bead array)

MP00876-3: 83981-4-PBS capture and 83981-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Securin fusion protein Ag12670
• Full Name: pituitary tumor-transforming 1
• Calculated Molecular Weight: 202 aa, 22 kDa
• GenBank Accession Number: BC101834
• Gene Symbol: Securin
• Gene ID (NCBI): 9232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.