SUN2 Polyclonal antibody, PBS Only

• 

  WB analysis using 27556-1-AP (same clone as 27556-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 27556-1-AP (same clone as 27556-1-PBS)

  WB result of SUN2 antibody (27556-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUN2 transfected HeLa cells. This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 27556-1-AP (same clone as 27556-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 27556-1-AP (same clone as 27556-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUN2 antibody (27556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27556-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SUN2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 27556-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

UNC84B, SUN domain-containing protein 2, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B

---

Product Information

27556-1-PBS targets SUN2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUN2 fusion protein Ag16077
• Full Name: unc-84 homolog B (C. elegans)
• Calculated Molecular Weight: 717 aa, 80 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC111717
• Gene Symbol: SUN2
• Gene ID (NCBI): 25777
• RRID: AB_2880906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UH99
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).