SUN2 Polyclonal antibody

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  WB analysis using 27556-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27556-1-AP

  WB result of SUN2 antibody (27556-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUN2 transfected HeLa cells.

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  WB analysis of mouse testis using 27556-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUN2 antibody (27556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

SUN2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 27556-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

UNC84B, SUN domain-containing protein 2, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

27556-1-AP targets SUN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUN2 fusion protein Ag16077
• Full Name: unc-84 homolog B (C. elegans)
• Calculated Molecular Weight: 717 aa, 80 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC111717
• Gene Symbol: SUN2
• Gene ID (NCBI): 25777
• RRID: AB_2880906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UH99
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).

Publications

Species	Application	Title
human	WB	Cancer Cell; Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite; Authors - Yihong Li
human	WB	Virology; Long non-coding RNA SUN2-AS1 acts as a negative regulator of ISGs transcription to promote flavivirus infection; Authors - Chao Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nuo (Verified Customer) (01-28-2023)

There are several non specific bands nearby.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

SUN2 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27556-1-AP)