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  • Featured Product
  • KD/KO Validated

SUN2 Polyclonal antibody

SUN2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27556-1-AP

Synonyms

UNC84B, SUN domain-containing protein 2, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUN2 Polyclonal antibody (27556-1-AP)

    WB analysis using 27556-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SUN2 Polyclonal antibody (27556-1-AP)

    WB analysis of HeLa using 27556-1-AP

    WB result of SUN2 antibody (27556-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUN2 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse testis tissue using SUN2 Polyclonal antibody (27556-1-AP)

    WB analysis of mouse testis using 27556-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUN2 Polyclonal antibody (27556-1-AP)

    IF Staining of HeLa using 27556-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUN2 antibody (27556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, Jurkat cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

27556-1-AP targets SUN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUN2 fusion protein Ag16077
Full Name unc-84 homolog B (C. elegans)
Calculated Molecular Weight 717 aa, 80 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC111717
Gene Symbol SUN2
Gene ID (NCBI) 25777
RRIDAB_2880906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SUN2 antibody 27556-1-APDownload protocol
IF protocol for SUN2 antibody 27556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite

Authors - Yihong Li
View Article
humanWB

Virology

Long non-coding RNA SUN2-AS1 acts as a negative regulator of ISGs transcription to promote flavivirus infection

Authors - Chao Yang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Nuo (Verified Customer) (01-28-2023)

There are several non specific bands nearby.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
SUN2 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27556-1-AP)