Biotin Plus-conjugated STXBP1 Monoclonal antibody

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  IHC staining of mouse brain using Biotin-67137

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67137 (STXBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using Biotin-67137

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67137 (STXBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

STXBP1 Monoclonal Antibody for IHC

Cat No. Biotin-67137

Clone No.1B5B3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IHC

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-67137 targets STXBP1 in IHC applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STXBP1 fusion protein Ag28605
• Full Name: syntaxin binding protein 1
• Calculated Molecular Weight: 594 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC015749
• Gene Symbol: STXBP1
• Gene ID (NCBI): 6812
• Conjugate: Biotin Plus
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61764
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.