STXBP1 Monoclonal antibody

STXBP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B5B3

Cat no : 67137-1-Ig

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

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Validation Data Gallery

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  WB analysis using 67137-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 67137-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67137-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67137-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67137-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67137-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67137-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67137-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 67137-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67137-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67137-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STXBP1 (67137-1-Ig, Clone:1B5B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, Y79 cells, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67137-1-Ig targets STXBP1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STXBP1 fusion protein Ag28605
• Full Name: syntaxin binding protein 1
• Calculated Molecular Weight: 594 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC015749
• Gene Symbol: STXBP1
• Gene ID (NCBI): 6812
• RRID: AB_2882436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.

Publications

Species	Application	Title
human	WB	BMC Biol; Identification of residues critical for the extension of Munc18-1 domain 3a; Authors - Xianping Wang