STXBP1 Monoclonal antibody, PBS Only

STXBP1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5B3

Cat no : 67137-1-PBS

Synonyms

EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

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Validation Data Gallery

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  WB analysis using 67137-1-Ig (same clone as 67137-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 67137-1-Ig (same clone as 67137-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67137-1-Ig (same clone as 67137-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STXBP1 (67137-1-Ig, Clone:1B5B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67137-1-PBS targets STXBP1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STXBP1 fusion protein Ag28605
• Full Name: syntaxin binding protein 1
• Calculated Molecular Weight: 594 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC015749
• Gene Symbol: STXBP1
• Gene ID (NCBI): 6812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.