CoraLite® Plus 488-conjugated STMN2 Monoclonal antibody

STMN2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F6C4

Cat no : CL488-67204

Synonyms

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

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Validation Data Gallery

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  IF Staining of SH-SY5Y using CL488-67204

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 STMN2 antibody (CL488-67204, Clone: 1F6C4 ) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL488-67204

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated STMN2 Monoclonal antibody (CL488-67204, Clone:1F6C4)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67204 targets STMN2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STMN2 fusion protein Ag28990
• Full Name: stathmin-like 2
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006302
• Gene Symbol: STMN2
• Gene ID (NCBI): 11075
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.