CoraLite® Plus 488-conjugated STK3 Monoclonal antibody

• Featured Product
• KD/KO Validated

STK3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C11A4

Cat no : CL488-66637

Synonyms

MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1, EC:2.7.11.1

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL488-66637

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 STK3 antibody (CL488-66637, Clone: 1C11A4 ) at dilution of 1:200.

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  FC experiment of NIH/3T3 using CL488-66637

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human STK3 (CL488-66637, Clone:1C11A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66637 targets STK3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STK3 fusion protein Ag2741
• Full Name: serine/threonine kinase 3 (STE20 homolog, yeast)
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 56-63 kDa
• GenBank Accession Number: BC010640
• Gene Symbol: STK3
• Gene ID (NCBI): 6788
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.