STK3 Monoclonal antibody

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• KD/KO Validated

STK3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse , Rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1C11A4

Cat no : 66637-1-Ig

Synonyms

KRS1, MST 2, MST2, STE20 like kinase MST2, STK3

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Validation Data Gallery

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  WB analysis of HeLa using 66637-1-Ig

  WB result of STK3 antibody (66637-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK3 transfected HeLa cells.

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  WB analysis using 66637-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66637-1-Ig (STK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 66637-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using STK3 antibody (66637-1-Ig, Clone: 1C11A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

66637-1-Ig targets STK3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STK3 fusion protein Ag2741
• Full Name: serine/threonine kinase 3 (STE20 homolog, yeast)
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 56-63 kDa
• GenBank Accession Number: BC010640
• Gene Symbol: STK3
• Gene ID (NCBI): 6788
• RRID: AB_2881996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Gen Subj; Extracellular matrix stiffness regulates degradation of MST2 via SCF βTrCP; Authors - Ana Paula Zen Petisco Fiore
human	WB,IF	Oncogene; Identification of a novel alternative splicing isoform of the Hippo kinase STK3/MST2 with impaired kinase and cell growth suppressing activities; Authors - Ana Maria Rodrigues