STIP1 Monoclonal antibody, PBS Only

STIP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5H2

Cat no : 68155-1-PBS

Synonyms

HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1

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Validation Data Gallery

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  WB analysis using 68155-1-Ig (same clone as 68155-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68155-1-Ig (STIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

  IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 68155-1-Ig (same clone as 68155-1-PBS)

  IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with Jurkat cells lysate 2400 ug. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STIP1 antibody (68155-1-Ig, Clone: 1B5H2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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Product Information

68155-1-PBS targets STIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STIP1 fusion protein Ag7851
• Full Name: stress-induced-phosphoprotein 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC002987
• Gene Symbol: STIP1
• Gene ID (NCBI): 10963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).