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STIP1 Monoclonal antibody

STIP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B5H2

Cat no : 68155-1-Ig

Synonyms

HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STIP1 Monoclonal antibody (68155-1-Ig)

    WB analysis using 68155-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68155-1-Ig (STIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunoprecipitation (IP) experiment of Jurkat cells using STIP1 Monoclonal antibody (68155-1-Ig)

    IP experiment of Jurkat using 68155-1-Ig

    IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with Jurkat cells lysate 2400 ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using STIP1 Monoclonal antibody (68155-1-Ig)

    IP experiment of HeLa using 68155-1-Ig

    IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with HeLa cells lysate 1880 ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using STIP1 Monoclonal antibody (68155-1-Ig)

    IHC staining of mouse liver using 68155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using STIP1 Monoclonal antibody (68155-1-Ig)

    IHC staining of mouse liver using 68155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STIP1 Monoclonal antibody (68155-1-Ig)

    IF Staining of HeLa using 68155-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STIP1 antibody (68155-1-Ig, Clone: 1B5H2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells, Jurkat cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68155-1-Ig targets STIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STIP1 fusion protein Ag7851
Full Name stress-induced-phosphoprotein 1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight61 kDa
GenBank Accession NumberBC002987
Gene Symbol STIP1
Gene ID (NCBI) 10963
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).

Protocols

Product Specific Protocols
WB protocol for STIP1 antibody 68155-1-IgDownload protocol
IHC protocol for STIP1 antibody 68155-1-IgDownload protocol
IF protocol for STIP1 antibody 68155-1-IgDownload protocol
IP protocol for STIP1 antibody 68155-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols