CoraLite® Plus 750-conjugated STIM1 Recombinant antibody

STIM1 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1B13

Cat no : CL750-81156

Synonyms

D11S4896E, GOK, STIM1, stromal interaction molecule 1

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Validation Data Gallery

• 

  WB analysis using CL750-81156

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-81156 (STIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-81156 targets STIM1 in WB applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: STIM1 fusion protein Ag2140
• Full Name: stromal interaction molecule 1
• Calculated Molecular Weight: 685 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC021300
• Gene Symbol: STIM1
• Gene ID (NCBI): 6786
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.