CoraLite® Plus 750-conjugated STIM1 Recombinant antibody

STIM1 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1B13

Cat no : CL750-81156

Synonyms

D11S4896E, GOK, STIM1, stromal interaction molecule 1



Tested Applications

Positive WB detected inNIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-81156 targets STIM1 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STIM1 fusion protein Ag2140
Full Name stromal interaction molecule 1
Calculated Molecular Weight 685 aa, 77 kDa
Observed Molecular Weight77 kDa
GenBank Accession NumberBC021300
Gene Symbol STIM1
Gene ID (NCBI) 6786
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 STIM1 antibody CL750-81156Download protocol
Standard Protocols
Click here to view our Standard Protocols