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SRP54 Monoclonal antibody, PBS Only

SRP54 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6D1

Cat no : 67005-1-PBS

Synonyms

SRP54

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRP54 Monoclonal antibody (67005-1-Ig)

    WB analysis using 67005-1-Ig (same clone as 67005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67005-1-Ig (SRP54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRP54 Monoclonal antibody (67005-1-Ig)

    IHC staining of human breast cancer using 67005-1-Ig (same clone as 67005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67005-1-Ig (SRP54 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRP54 Monoclonal antibody (67005-1-Ig)

    IHC staining of human breast cancer using 67005-1-Ig (same clone as 67005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67005-1-Ig (SRP54 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67005-1-PBS targets SRP54 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SRP54 fusion protein Ag12166
Full Name signal recognition particle 54kDa
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC003389
Gene Symbol SRP54
Gene ID (NCBI) 6729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).