CoraLite®594-conjugated SRF Monoclonal antibody

• Featured Product
• KD/KO Validated

SRF Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G8F4

Cat no : CL594-66742

Synonyms

MCM1, Serum response factor, SRF

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66742

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66742 (SRF antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66742 targets SRF in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SRF fusion protein Ag10386
• Full Name: serum response factor (c-fos serum response element-binding transcription factor)
• Calculated Molecular Weight: 508 aa, 52 kDa
• GenBank Accession Number: BC048211
• Gene Symbol: SRF
• Gene ID (NCBI): 6722
• RRID: AB_2883622
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).