SRF Monoclonal antibody

• Featured Product
• KD/KO Validated

SRF Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1G8F4

Cat no : 66742-1-Ig

Synonyms

Serum response factor, MCM1, 1G8F4

---

Validation Data Gallery

• 

  WB analysis using 66742-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66742-1-Ig

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.

• 

  WB analysis using 66742-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66742-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66742-1-Ig

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected HeLa cells.

• 

  IF Staining of HepG2 using 66742-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66742-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66742-1-Ig (SRF antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells, MCF-7 cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, SK-BR-3 cells, IMR-32 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

66742-1-Ig targets SRF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SRF fusion protein Ag10386
• Full Name: serum response factor (c-fos serum response element-binding transcription factor)
• Calculated Molecular Weight: 508 aa, 52 kDa
• GenBank Accession Number: BC048211
• Gene Symbol: SRF
• Gene ID (NCBI): 6722
• RRID: AB_2882090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis; Authors - Ni Zeng
human	WB	bioRxiv; Single molecule imaging of the central dogma reveals myosin-2A gene expression is regulated by contextual translational buffering; Authors - O'Neil Wiggan ; KD Validated
mouse	WB	Mol Neurobiol; SRF Facilitates Transcriptional Inhibition of Gem Expression by m6A Methyltransferase METTL3 to Suppress Neuronal Damage in Epilepsy; Authors - Lianling Li