SRF Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SRF Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G8F4

Cat no : 66742-1-PBS

Synonyms

MCM1, Serum response factor, SRF

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Validation Data Gallery

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  WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66742-1-Ig (same clone as 66742-1-PBS)

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66742-1-Ig (same clone as 66742-1-PBS)

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected HeLa cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66742-1-Ig (same clone as 66742-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66742-1-Ig (SRF antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66742-1-PBS targets SRF in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SRF fusion protein Ag10386
• Full Name: serum response factor (c-fos serum response element-binding transcription factor)
• Calculated Molecular Weight: 508 aa, 52 kDa
• GenBank Accession Number: BC048211
• Gene Symbol: SRF
• Gene ID (NCBI): 6722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).