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  • Featured Product
  • KD/KO Validated

SRF Monoclonal antibody

SRF Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8F4

Cat no : 66742-1-Ig

Synonyms

Serum response factor, MCM1, 1G8F4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis using 66742-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis of MCF-7 using 66742-1-Ig

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis using 66742-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis of RAW 264.7 using 66742-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis of HeLa using 66742-1-Ig

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SRF Monoclonal antibody (66742-1-Ig)

    IF Staining of HepG2 using 66742-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SRF Monoclonal antibody (66742-1-Ig)

    IF Staining of HepG2 using 66742-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66742-1-Ig (SRF antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, MCF-7 cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, SK-BR-3 cells, IMR-32 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66742-1-Ig targets SRF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SRF fusion protein Ag10386
Full Name serum response factor (c-fos serum response element-binding transcription factor)
Calculated Molecular Weight 508 aa, 52 kDa
GenBank Accession NumberBC048211
Gene Symbol SRF
Gene ID (NCBI) 6722
RRIDAB_2882090
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Protocols

Product Specific Protocols
WB protocol for SRF antibody 66742-1-IgDownload protocol
IF protocol for SRF antibody 66742-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis

Authors - Ni Zeng
View Article
humanWB

bioRxiv

Single molecule imaging of the central dogma reveals myosin-2A gene expression is regulated by contextual translational buffering

Authors - O'Neil Wiggan
  • KD Validated
View Article
mouseWB

Mol Neurobiol

SRF Facilitates Transcriptional Inhibition of Gem Expression by m6A Methyltransferase METTL3 to Suppress Neuronal Damage in Epilepsy

Authors - Lianling Li
View Article