SOX2 Monoclonal antibody, PBS Only

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  WB analysis using 66411-1-Ig (same clone as 66411-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66411-1-Ig (same clone as 66411-1-PBS)

  WB result of SOX2 antibody (66411-1-Ig, 1:4000) with si-Control and si-SOX2 transfected HEK293 cells.. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

  WB result of SOX2 antibody (66411-1-Ig; 1:3000; room temperature for 1.5 hours) with negative control and SOX2 knockout U-251 cells. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66411-1-Ig (same clone as 66411-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66411-1-Ig (same clone as 66411-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66411-1-Ig (same clone as 66411-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66411-1-Ig (same clone as 66411-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

  1X10^6 U-251 cells were intracellularly stained with 0.5 ug Anti-Human SOX2 (66411-1-Ig, Clone:4B9B10) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SOX2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 66411-1-PBS

Clone No.4B9B10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

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Product Information

66411-1-PBS targets SOX2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOX2 fusion protein Ag13635
• Full Name: SRY (sex determining region Y)-box 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC013923
• Gene Symbol: SOX2
• Gene ID (NCBI): 6657
• RRID: AB_2881783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P48431
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Background

SRY (sex determining region Y)-box 2 is a transcription factor involved in neural stem cell maintenance and has been used in somatic cell reprogramming.

 What is the molecular weight of Sox2?

34 kDa. Sox2 is an intronless gene encoding xx amino acids including HMG (High-mobility group) box domains that can bind to DNA.

 What is the tissue specificity of Sox2?

In embryonic stem cells, Sox2 is highly expressed and is essential in self-renewal. In the developing embryo, Sox2 is found in the neural tube and then in the developing eye and central nervous system (CNS). This expression is highly conserved across organisms (PMID: 25126380; PMID: 8625802).

In adult tissues Sox2 is expressed in neural progenitor cells, which as multipotent cells can both self-renew and differentiate into neurons, astroglia, and oligodendroglia. It maintains their stem cell phenotype and is a useful marker of early neural stem cells (PMID: 16139372).

 What is the role of Sox2 in pluripotency?

Sox2 acts as part of the LIF (Leukemia inhibitory factor) pathway, activated via JAK-STAT signaling to form complexes with other transcription factors such as Oct4 that bind to DNA and work to control pluripotency. Sox2 was described as one of the four original factors that are key to the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) (PMID: 16904174).

 What is the role of Sox2 in cancer?

The ability to control pluripotency means dysregulation of Sox2 can lead to uncontrolled proliferation. In many different cancers, Sox2 has been associated with the metastasis and growth of tumors, mostly due to overexpression (PMID: 28388544). The increased levels of Sox2 RNA and protein in tumor tissue highlight it as a potential therapeutic target (PMID: 25114775).