SOX2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SOX2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4B9B10

Cat no : 66411-1-PBS

Synonyms

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

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Validation Data Gallery

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  WB analysis of HEK-293 using 66411-1-Ig (same clone as 66411-1-PBS)

  WB result of SOX2 antibody (66411-1-Ig, 1:4000) with si-Control and si-SOX2 transfected HEK293 cells.. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  WB analysis using 66411-1-Ig (same clone as 66411-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

  WB result of SOX2 antibody (66411-1-Ig; 1:3000; room temperature for 1.5 hours) with negative control and SOX2 knockout U-251 cells. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66411-1-Ig (same clone as 66411-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66411-1-Ig (same clone as 66411-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66411-1-Ig (same clone as 66411-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66411-1-Ig (same clone as 66411-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

  1X10^6 U-251 cells were intracellularly stained with 0.5 ug Anti-Human SOX2 (66411-1-Ig, Clone:4B9B10) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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Published Applications

• IF: See 1 publications below

Product Information

66411-1-PBS targets SOX2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOX2 fusion protein Ag13635
• Full Name: SRY (sex determining region Y)-box 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC013923
• Gene Symbol: SOX2
• Gene ID (NCBI): 6657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2.

Publications

Species	Application	Title
rat	IF	Neural Regen Res; Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions; Authors - Yuxue Mu