SOD2 Recombinant antibody
SOD2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240462D8
Cat no : 83519-3-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells, SH-SY5Y cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HUVEC cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83519-3-RR is SOD2 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SOD2 fusion protein Ag21388 |
Full Name | superoxide dismutase 2, mitochondrial |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC016934 |
Gene Symbol | SOD2 |
Gene ID (NCBI) | 6648 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for SOD2 antibody 83519-3-RR | Download protocol |
IHC protocol for SOD2 antibody 83519-3-RR | Download protocol |
IF protocol for SOD2 antibody 83519-3-RR | Download protocol |
FC protocol for SOD2 antibody 83519-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |