SOD2 Recombinant antibody, PBS Only

SOD2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240462D8

Cat no : 83519-3-PBS

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

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Validation Data Gallery

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  WB analysis of HepG2 using 83519-3-RR (same clone as 83519-3-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 83519-3-RR (SOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83519-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83519-3-RR (same clone as 83519-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-3-RR (SOD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83519-3-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 83519-3-RR (same clone as 83519-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (83519-3-RR, Clone: 240462D8 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83519-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83519-3-RR (same clone as 83519-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOD2 Recombinant antibody (83519-3-RR, Clone:240462D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83519-3-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 83519-3-PBS is SOD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SOD2 fusion protein Ag21388
• Full Name: superoxide dismutase 2, mitochondrial
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016934
• Gene Symbol: SOD2
• Gene ID (NCBI): 6648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.